妊娠期糖尿病孕妇与健康孕妇的肠道菌群差异

摘要： 目的·探讨妊娠期糖尿病（gestational diabetes mellitus，GDM）孕妇与健康孕妇之间肠道菌群的差异。方法·招募2017年8月—2019年1月于上海交通大学医学院附属新华医院产科产检孕妇74例，其中GDM孕妇（GDM组）51例、健康孕妇（健康组）23例，留取所有孕妇的新鲜粪便样本。利用Illumina MiSeq平台对2组样本细菌的基因序列进行高通量测序，再采用线性判别分析（linear discriminant analysis，LDA）、组间群落差异分析（LDA EffectSize，LEfSe）分别对2组粪便样本的菌群进行肠道菌群α多样性及表达丰度差异检验。结果·与健康组相比，GDM组孕妇的肠道菌群丰度更高（P0.027），在科级别上紫单胞菌科（Porphyromonadaceae）、产碱菌科（Alcaligenaceae）丰度更高，在属级别上拟杆菌属（Bacteroides）丰度更高（均PNeisseria）、链球菌属（Streptococcus）、产丁酸菌（Butyricicoccus）、加德纳菌属（Gardnerella）、奇异菌属（Atopobium）丰度更高（均P结论·GDM孕妇的肠道菌群与健康孕妇间存在较为明显的差异。

关键词: 妊娠期糖尿病, 肠道菌群, 高通量测序, &, alpha, 多样性

Abstract:

Objective · To investigate the differences of intestinal flora between gestational diabetes mellitus (GDM) pregnant women and healthy pregnant women. Methods · In this study, 74 pregnant women the Department of Obstetrics in Xinhua Hospital, Shanghai Jiao Tong University School of Medicine Aug. 2017 to Jan. 2019 were recruited, including 51 GDM pregnant women (GDM group) and 23 healthy pregnant women (healthy group). High-throughput sequencing was performed on bacterial gene sequences the fresh feces of all pregnant womenusing Illumina MiSeq platform. Linear discriminant analysis (LDA) and LDA EffectSize (LEfSe) were used to detect the α diversity and abundance of intestinal flora of the two groups. Results · Compared with the healthy group, the intestinal flora richness of pregnant women in the GDM group was significantly higher (P0.027). Porphyromonadaceae and Alcaligenaceae in Family, and Bacteroides in Genus of microbiota in the GDM group were significantly higher (all P in Order, Lachnospirace in Family, and Neisseria, Streptococcus, Butyricicoccusm, Gardnerella and Atopobium in Genus of microbiota in the healthy group were significantly higher (all PConclusion · There are significant differences of intestinal flora between GDM pregnant women and healthy pregnant women.

Key words: gestational diabetes mellitus (GDM), intestinal flora, high-throughput sequencing, &alpha, diversity

中图分类号: 

R714.7

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