绿茶茶渣半纤维素的提取及组成性质研究

摘要: 目的:从绿茶茶渣中分离半纤维素,研究其组成性质。方法:利用碱性过氧化氢法浸提、乙醇沉淀提取得到茶渣半纤维素。红外光谱法测定其糖苷键类型;离子色谱法分析其单糖组成;高效凝胶色谱法测定其相对分子质量。结果:红外光谱中半纤维素具有糖类物质的特征吸收峰;茶渣半纤维素主要由葡萄糖、半乳糖、阿拉伯糖组成,另外还含有少量的鼠李糖、木糖和氨基半乳糖。高浓度乙醇沉淀的茶渣半纤维素相对分子量在2000左右;结论:该茶渣半纤维素是一种酸性半纤维素多糖。 

Abstract: Objective:To get hemicelluloses polysaccharide from green tea-leaf and study the composition of the polysaccharide. Methods:The hemicelluloses was obtained from tea-leaf that without most water soluble composition by ways as follows:Alkaline hydrogen peroxide extraction, graded ethanol precipitation. The form of glycosidic bond was determined by IR. The monosaccharide components of hemicelluloses were determined by Ion Chromatograph. Its MW was measured by HPGFC. Results:Typical absorption of sugar was shown in the IR spectrum. Glucose, galactose, arabinose, rhamnose, mannose and galactosamine. The MW of the hemicelluloses which were precipitated by high concentrations of ethanol was estimated 2000. Conclusion:The hemicelluloses extract by alkaline hydrogen peroxide was a kind of acidic hemicelluloses polysaccharide.

[1] 王黎明.具有降血糖活性的茶多糖组分分离纯化和结构鉴定[D].无锡:江南大学, 2006. [2]

Revanappa S B, Salimath P V.Structure characterization of hemicelluloses A from whea (tTriticum aestivum) vatieties differing in their chapatti-making quality[J].Carbohydrate Polymers, 2010, 79:655-659.

[3]

Pai P, Feng P, Jing B, et al.Isolation and structural characterization of hemicelluloses from bamboo species Phyllostachys incarnate Wen[J].Carbohydrate Polymers, 2011, 86:883-890.

[4]

Feng P, Jun-Li R, Feng X, et al.Fractional study of alkali-soluble hemicelluloses obtained by graded ethanol precipitation from sugar cane bagasse[J].Journal of Agricultural and Food Chmeistry, 2010, 58:1768-1776.

[5]

Yong-Chang S, Jia-Long W, Feng X, et al.Organosolv-and alkali-soluble hemincelluloses degraded from Tamarix austromongolica:Characterization of physicochemical, structural features and thermal stability[J].Polymer Degradation and Stability, 2011, 96:1478-1488.

[6]

Egues I, Sanchez C, Mondragon I, et al.Effect of alkaline and autohydrolysis processes on purity of obtained hemicelluloses from corn stalks[J].Bioresource Technology, 2012, 103:239-248.

[7]

Ebingerova A, Hromadkova Z, Heinze T.Hemicellulose[J].Advances in Polymer Science, 2005, 186:1-67.

[8] 史德芳, 杨洋, 任红.仙人掌多糖提取过程中三种脱蛋白方法的比较研究[J].山东食品发酵, 2006 (2) :42-44. [9]

Zhou X L, Sun P N, Bucheli P, et al.FT-IR methodology for quality control of arabinogalactan protein (AGP) extracted from green tea (Camellia sinensis) [J].Journal of Agricultural and Food Chemisty, 2009, 57:5121-5128.

[10] 戴军, 朱松, 汤坚.PMP柱前衍生高效液相色谱法分析杜氏盐藻多糖组成[J].分析测试学报, 2007 (2) :206-210. [11] 徐燕, 朱科学, 钱海峰, 等.桃胶多糖的提取分离机组成性质研究[J].食品工业科技, 2008 (1) :66-71. [12] 张惟杰.糖复合物生化研究技术[M].杭州:浙江大学出版社, 1999:10-11. [13] 张云贵, 刘祥云, 李天俊, 等.生物化学实验指导[M].北京:中国农业出版社, 2007-12. [14] 中华人民共和国农牧渔业部.NY82.11-1988果胶的测定[S].北京:中国标准出版社, 1988. [15] 彭锋.农林生物质半纤维素分离纯化、结构表征及化学改性的研究[D].广州:华南理工大学, 2010. [16]

Fang J M, Sun R C, Salisbury D, et al.Comparative study of hemicelluloses from wheat straw by alkali and hydrogen peroxide extractions[J].Polymer Degradation and Stability, 1999, 66:423-432.

[17] 蔚慰奇, 武书彬, 彭云云.麦草水溶性和碱溶性半纤维素的分离和表征[J].林产化学与工业, 2010 (6) :66-70.

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