响应面法优化山豆根多糖提取工艺及其分级醇沉组分的抗氧化活性

XU Hai-tang, LIAO Hua-zhen, ZHAO Yan-zhi, ZHANG Jin-yan, ZHOU Ju-ying. Optimization Extraction of Polysaccharide from Sophora tonkinensis Gagnep by Response Surface Methodology and Its Antioxidant Activity of Fractionated Alcohol Precipitation Components[J]. Science and Technology of Food Industry, 2019, 40(22): 157-162. DOI: 10.13386/j.issn1002-0306.2019.22.028

Citation: XU Hai-tang, LIAO Hua-zhen, ZHAO Yan-zhi, ZHANG Jin-yan, ZHOU Ju-ying. Optimization Extraction of Polysaccharide from Sophora tonkinensis Gagnep by Response Surface Methodology and Its Antioxidant Activity of Fractionated Alcohol Precipitation Components[J]. Science and Technology of Food Industry, 2019, 40(22): 157-162. DOI: 10.13386/j.issn1002-0306.2019.22.028

响应面法优化山豆根多糖提取工艺及其分级醇沉组分的抗氧化活性

许海棠,  廖华珍1,  赵彦芝1,  张金彦1,  周菊英1, , 

1. 广西民族大学化学化工学院, 广西林产化学与工程重点实验室, 广西南宁 530008;

2. 广西民族大学海洋与生物技术学院, 广西海洋微生物资源产业化工程技术研究中心, 广西南宁 530008

详细信息

作者简介:

许海棠(1975-),女,硕士,高级实验师,研究方向:天然药物资源的开发研究,E-mail:xhthellen@163.com。

通讯作者:

周菊英(1979-),女,博士,教授,研究方向:天然产物资源的研究与开发,E-mail:zhoujuying@126.com。

中图分类号: TS255.1

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Optimization Extraction of Polysaccharide from Sophora tonkinensis Gagnep by Response Surface Methodology and Its Antioxidant Activity of Fractionated Alcohol Precipitation Components

1. Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530008, China;

2. Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530008, China

摘要

摘要: 为研究山豆根多糖的提取工艺及其抗氧化性,采用热水浸提法提取山豆根粗多糖(SGP),研究提取温度、提取时间、液料比对多糖得率的影响,在单因素实验基础上采用响应面法对山豆根粗多糖的提取工艺进行条件优化。将提取得到的粗多糖分级醇沉,并分别采用清除DPPH、ABTS+自由基及还原能力的方法对各醇沉组分多糖的抗氧化活性进行评估。结果表明,山豆根粗多糖的最佳提取工艺条件为:提取温度83℃,提取时间133 min,液料比30:1 mL/g。在此工艺条件下,山豆根粗多糖得率为3.98%。粗多糖经分级醇沉共获得SGP50、SGP70、SGP80和SGP90 4个组分,且SGP90表现出最强的抗氧化能力,尤其是在清除DPPH自由基方面,显著高于其它组分(P<0.05)。

Abstract: To explore the extraction process and antioxidant activity of polysaccharide from Sophora tonkinensis Gagnep,the Sophora tonkinensis Gagnep was used for extracting polysaccharide with hot water. The effects of extraction temperature,extraction time and ratio of liquid to material on the extraction rate of polysaccharide were investigated. On the basis of single factor tests,the extraction technology of polysaccharide from Sophora tonkinensis Gagnep was optimized by response surface methodology(RSM). The extracted crude polysaccharide was precipitated and fractionated by ethanol,and the antioxidant activity of each alcohol component was evaluated by the methods of scavenging DPPH,ABTS+ radical and reducing ability,respectively. Results showed that,the optimum extraction conditions were as follows:The extraction temperature was 83 ℃,the extraction time was 133 min,the ratio of liquid to material was 30:1 mL/g. Under the optimal extraction conditions,the yield of polysaccharides from Sophora tonkinensis Gagnep was 3.98%. The crude polysaccharide obtained four components SGP50,SGP70,SGP80 and SGP90 by fractional alcohol precipitation. And the SGP90 showed the strongest antioxidant activity,especially in the DPPH free radical scavenging,which was significantly higher than other components(P<0.05).

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计量 文章访问数:  HTML全文浏览量:  PDF下载量:  出版历程 收稿日期:  2019-02-21 网络出版日期:  2020-11-12 刊出日期:  2019-11-14

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